Methylation analysis of EMILIN2 in colorectal cancer. (a) COBRA results are shown with undigested (-) PCR product next to digested (+) PCR product for matching normal (N) and colorectal carcinoma (C) in the upper panel and matching normal (N) and adenoma (A) in the lower panel. (b) Selected PCR products were cloned and sequenced. Individual alleles of each of the four samples shown are represented by a line, black and white circles represent methylated and unmethylated CpG dinucleotides respectively. The frequency of methylation within the amplified region for each sample is indicated by the methylation index (MI).
Hill et al. Molecular Cancer 2010 9:51 doi:10.1186/1476-4598-9-51