Figure 7.

A diagram depicting the change in HER2 expression and tumorigenicity of R2N1d (CD44⁺/CD24^- sorted) cells due to cell culture condition. Adherent R2N1d cells were OCT4⁺/HER2⁺ and highly tumorigenic (tumor developed in 1 month after inoculation), the non-adherent R2N1d cells detached from confluent R2N1d cells in suspension were OCT4⁺/HER2^- and took longer time (6 months) to develop tumor at lower frequency. The reattached R2N1d cells from non-adherent cells were similar to parental adherent cells in OCT4⁺/HER2⁺ expression and tumor development.