Expression of breast cancer stem cell markers CD44+/CD24-/low in R2d and R2N1d cells and Modulation of HER2, Oct-4, AKT and HDAC6 expression in R2N1 cells by cell culture condition. A, higher frequency of CD44+/CD24-/low cells was found in R2N1d cells than R2d cells (~50% vs. ~10%). R1 region denotes a small population of CD44high cells which was increased in R2N1d cells. The fluorescence cut off level: high expression was fluorescence intensity > 103; negative or low expression was fluorescence intensity < 102. B, CD44+/CD24- cells sorted by flow cytometry tend to show contact-insensitive growth in confluent culture and gave rise to non-adherent cells in suspension. By flow cytometric analysis (C, E), and by western blotting (D), adherent cells and re-attached cells were found to be HER2+/OCT4+/AKT+/HDAC6+, whereas non-adherent cells were HER2-/OCT4+/AKT -/HDAC6-. The length of the scale bar of these photos is 100 μm.
Wang et al. Molecular Cancer 2010 9:288 doi:10.1186/1476-4598-9-288