Figure 3.

HER2 effects on COX-2 and HDAC6 expression and cell invasion. A, the effect of AG825 (a HER2 tyrosine kinase inhibitor, 25 μM), U0126 (a highly selective inhibitor of MAPK/ERK kinase, 10 μM) or NS398 (a COX-2 inhibitor, 100 μM) treatment on COX-2 protein expression in R2N1d cells by flow cytometry analysis. B, the effect of AG825, U0126 or NS398 treatment on COX-1 and COX-2 expression in R2N1d cells by western blotting analysis. C, the expression of HDAC6 in R2d and R2N1d cells by flow cytometry analysis. D, the effect of AG825 (25 μM) and LY294002 (10 μM) (a PI3K inhibitor) treatment on HDAC6 expression in R2N1d cells by western blotting analysis. E, the effect of AG825 (25 μM) treatment for 24 hr on HER2 and HDAC6 expression in R2d and R2N1d cells by RT-PCR analysis. F, the effect of HER2 inhibitor, AG825 (25 μM), treatment for 24 hr on invasion ability of R2N1d cells, assayed by using the invasion chamber. R2d cells were also included in the experiment.

Wang et al. Molecular Cancer 2010 9:288   doi:10.1186/1476-4598-9-288
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