Figure 2.

siRNA-mediated depletion of CBFβ in MDA-MB-231 cells inhibits their invasive capacity. (A) Western blot showing siRNA knockdown of CBFβ. The upper panel shows total cell lysates derived from siRNA transfections of MDA-MB-231 cells after immunodetection with an anti-CBFβ antibody. The lower panel is a Tubulin loading control. (B) MDA-MB-231 cells were transfected with siRNA against CBFβ (siCBFβ) and a non-specific siRNA (siNS). 24 hrs after a second transfection the cells were plated on Matrigel for invasion assays. Wild type (WT) MDA-MB-231 cells were used as positive control for invasion. Cells that migrated were stained and counted in random fields. Data are presented as mean ± standard deviation (S.D.) (n = 3). * indicates p < 0.05 compared with siNS by analysis of variance. (C) Western blot showing siRNA knockdown of Runx2. The upper panel shows total cell lysates derived from siRNA transfections of MDA-MB-231 cells after immunodetection with an anti-Runx2 antibody. The lower panel is a Tubulin loading control. (D) Runx2 is required for invasion of MDA-MB-231 cells. MDA-MB-231 cells were transfected with siRNA against Runx2 (siRunx2) or a non-specific siRNA (siNS). 24 hrs after a second transfection the cells were plated on Matrigel for invasion assays. Wild type (WT) MDA-MB-231 cells were used as a positive control for invasion. Cells that migrated were stained and counted in random fields. Data are presented as mean ± standard deviation (S.D.) (n = 3). * indicates p < 0.05 compared with siNS by analysis of variance.