Comparative analysis of noncanonical (top) and unclassified (bottom) Wnt ligands in hepatocellular carcinoma cell lines. Noncanonical and unclassified Wnt ligands were tested for expression by RT-PCR assay. Total RNAs were extracted from cell lines and used to detect gene expression by RT-PCR assay. See Figure 1 for GAPDH loading control.
Yuzugullu et al. Molecular Cancer 2009 8:90 doi:10.1186/1476-4598-8-90