Figure 5.

Analysis of luciferase stability and function in vivo. A. – Intrahepatic MC38Luc1 tumors (n = 10) were excised at different times after cell implantation (2, 3 and 4 weeks), and the relative copy number of the plasmid pCDNA3-luc was determined by qPCR. The values correspond to the number of plasmid copies divided by the copies of the endogenous albumin gene. Error bars represent standard deviations. B. – Correlation between the relative pCDNA3-luc copy number in advanced tumors (4 weeks after implantation) and tumor volume. C. – Correlation between in vivo light emission of advanced tumors (in photons/sec.) and luciferase activity (in RLUs) obtained in solution from tumor extracts (log₁₀ conversion of values).