Figure 3.

Correlation between BLI, US and direct tumor volume determination in hepatic tumors. MC38Luc1 cells (5 × 10⁵) were injected in the liver of C57BL/6 mice (n = 20), and tumor progression was evaluated weekly by either BLI, US or direct tumor measurement through laparotomy or necropsy. A – Tumor volume determined by direct measurement (calliper, black circles) or US (black squares) over time. Volumes are indicated in the left Y axis. Light emission quantified by BLI is represented as a dotted line and the scale corresponds to (photons/s) × 10³ in the right Y axis. Error bars represent standard deviations. B.-Correlation between tumor volumes determined by US (X axis) and direct measurement after necropsy (calliper, Y axis) one month after cell implantation. C-D – Correlation between light emission and direct tumor measurement 2 weeks (C) or 3 weeks (D) after cell implantation (log₁₀ conversion of values).