PRL-3 promotes the motility, invasion, and metastasis of LoVo colon cancer cells through PRL-3-integrin β1-ERK1/2 and-MMP2 signaling

doi:10.1186/1476-4598-8-110

Molecular Cancer

Volume 8

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PRL-3 promotes the motility, invasion, and metastasis of LoVo colon cancer cells through PRL-3-integrin β1-ERK1/2 and-MMP2 signaling

Lirong Peng^* , Xiaofang Xing^* , Weijun Li^* , Like Qu , Lin Meng , Shenyi Lian , Beihai Jiang , Jian Wu and Chengchao Shou

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, PR China

author email corresponding author email* Contributed equally

Molecular Cancer 2009, 8:110doi:10.1186/1476-4598-8-110


Published:	24 November 2009

Additional files

Additional file 1:

Western blot analysis of PRL-3 protein in LoVo cells. LoVo-C and LoVo-P cells were treated with 2 μm Adriamycin or DMSO for 12 h, then cells were harvested and cell lysates (50 μg of protein) were subjected to Western blot with antibodies against PRL-3 (Sigma), Myc, p53 (DO-1, Santa Cruz), and GAPDH, respectively. Antibody to PRL-3 recognized exogenous PRL-3, but no endogenous PRL-3 was detected in both cell lines. LoVo-P cells had more p53 expression, which was consistent with results from Basak et al [Ref [23]].

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Additional file 2:

Wound healing assay. The effect of PRL-3 on the motilities of LoVo cells analyzed with wound healing assay for 72 h and the closure of the wound line was recorded every 24 h.

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Additional file 3:

Correlation between Expression of PRL-3 and P-ERK1/2 in Human Colon Cancer Tissues. File shows the correlation between expression of PRL-3 and P-ERK1/2 in human colon cancer tissues.

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