Inhibition of EGFR transport into the cytoplasm/nucleus by src siRNA. Exponentially growing A549 cells were incubated either with control- or src-specific siRNA for 72 h at a concentration of 30 nM. Subsequently confluent cells were irradiated with 4 Gy and cytoplasmic (A) and nuclear proteins (B) were isolated at the time points given. Corresponding results were presented for FaDu cells (C and D). Expression of specific proteins was quantified by densitometry, normalized either to actin or lamin B1 and shown as fold-induction relative to untreated control. Experiments were performed three times; shown are representative results.
Dittmann et al. Molecular Cancer 2008 7:69 doi:10.1186/1476-4598-7-69