Stabilization of caveolin1/EGFR complex in cytoplasm after incubation with EGFR inhibitory antibody Erbitux. Confluent A549 cells were treated with human IgG or EGFR inhibitory antibody Erbitux at a concentration of 30 nM for 1 h. Subsequently cells were irradiated with 4 Gy and cytoplasmic and nuclear proteins were isolated at time points given. Proteins were separated by SDS-PAGE and after blotting EGFR protein was quantified with help of a specific antibody. Experiments were performed three times; shown are representative results. Equal protein input for immune-precipitation was documented by showing actin expression within aliquots of input proteins. Expression of specific proteins was quantified by densitometry and shown as fold-induction relative to untreated control.
Dittmann et al. Molecular Cancer 2008 7:69 doi:10.1186/1476-4598-7-69