  ResearchSoluble histone H2AX is induced by DNA replication stress and sensitizes cells to undergo apoptosisYing Liu1 1Molecular Virology Program, University of Pittsburgh Cancer Institute, Pittsburgh, PA, 15213, USA 2Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15261, USA 3Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15261, USA 4University of Pittsburgh Cancer Institute, Hillman Cancer Center, Research Pavilion Suite 1.8, 5117 Centre Avenue, Pittsburgh, PA, 15213, USA
Molecular Cancer 2008, 7:61doi:10.1186/1476-4598-7-61
Additional filesAdditional File 1: DNA replication stress induces soluble H2AX in H1299 lung carcinoma cells. Immunoblot analysis of soluble nuclear (S3) and chromatin bound (P3) protein fractions obtained from H1299 cells transiently transfected with empty GFP vector, GFP-H2AX or GFP-H2AX-S139A and treated with 10 μM aphidicolin (APH) for 1 h to block DNA replication. Staining for GFP detects expression of tagged proteins, whereas staining for H2AX detects endogenous protein. Immunoblot for ORC2 is shown to rule out carry-over between fractions. Ponceau S staining demonstrates protein loading. Format: TIFF Size: 1MB Download file |
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