Figure 5.

HDACi increase the pro- to anti-apoptotic protein ratio by different mechanisms. A. SH-EP cells were unstimulated (no), or stimulated with NaB (20 mM), SAHA (20 μM) or TSA (5 μM) in the absence (-) or in the presence (+) of zVAD-fmk for 48 h. IMR32 cells were unstimulated (no) or stimulated with 2.5 μM of SAHA in the absence (-) or in the presence (+) of zVAD-fmk for 48 h. Whole cell extracts were analysed by immunoblotting with anti-Bid, Bim, XIAP, RIP, Bcl-x_L survivin antibodies. B. SH-EP cells were unstimulated (no), or stimulated with NaB, SAHA or TSA in the absence (-) or in the presence (+) of Lactacystin (10 μM) as in (A). IMR32 were unstimulated (no) or stimulated with SAHA (5 μM) in the absence (-) or in the presence (+) of Lactacystin (10 μM) for 48 h. Whole cell extracts were analysed for the presence of survivin. β-actin was used as loading control (A and B).