Additional file 1.

Effect of hypoxia and/or etoposide on protein abundance and subcellular localization of different transcription factors. Cells were incubated under normoxic (N) or hypoxic (H) conditions with or without etoposide (e) at 50 μM for 5 hours. After the incubation, cells were fixed, permeabilized and stained for HIF-1α, p53, phospho-serine 15 p53, c-jun and phospho-serine 63 c-jun, using specific antibodies (green). Nuclei were detected with To-Pro-3 (blue). Observation was performed using a confocal microscope with the photomultiplier constant. Please refer to supplementary data (fig. 3.S) for results obtained for c-fos, c-myc, phospho-elk, phospho-ATF-2, phospho-CREB, STAT-1α, p65, p50, c-rel and MEF-2. Primary antibodies were as follows: mouse anti-p53 (#05-224 Upstate), mouse anti-phospho serine 15-p53 (#92865 Cell Signaling), rabbit anti-c-jun (SC-1694 Santa Cruz), rabbit anti-phospho serine 63-c-jun (#92615 Cell Signaling), rabbit anti-c-fos (SC-052 Santa Cruz), mouse anti-phospho serine 83-elk-1 (SC-8406 Santa-Cruz), mouse anti-phospho threonine 71-ATF2 (SC-8398 Santa Cruz), rabbit anti-phospho serine 133-CREB (#06-519 Upstate), rabbit anti-MEF-2 (SC-10794 Santa Cruz), mouse anti-c-myc (SC-42 Santa Cruz), rabbit anti-STAT-1α (SC-345 Santa Cruz), mouse anti-c-rel (SC-6955 Santa Cruz), rabbit anti-p50 (SC-7178 Santa Cruz), rabbit anti-p65 (SC-372 Santa Cruz), mouse anti-HIF-1α (#610958 BD Biosciences).

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Sermeus et al. Molecular Cancer 2008 7:27   doi:10.1186/1476-4598-7-27