Figure 2.

Effect of PSAT1 overexpression on cell proliferation and tumor xenograft. A, Western blot analysis of PSAT1 expression in SW480-FlpIn and SW480-PSAT1 cell lines using anti-PSAT1 or anti-V5 antibodies. B, Growth curve of SW480-FlpIn and SW480-PSAT1 cultured in low serum medium (1%). The curves are representative of three experiments. C, Representative nude mice, SW480-FlpIn were injected subcutaneously into the left flank and SW480-PSAT1 into the right flank. D, Tumor volume evolution after a subcutaneous xenograft of SW480-FlpIn and SW480-PSAT1 in 7 athymic nude mice. E, In vivo tumorigenic frequency for SW480-FlpIn and SW480-PSAT cell lines. Ratios indicate the number of mice presenting an observable tumor compared with the total number of mice observed. Numbers in parentheses indicate percentages.