Figure 4.

Use of shRNA constructs to knock-down Fra-1 confirm Fra-1-dependent CD44 expression in MMs. A. Western blots of SV40- (MM1) and SV40+ (MM3) lines showing empty vector (EV) controls or RNAi shFra-1 constructs. B. Immunofluorescence image showing CD44 (red) levels MM cell lines. C. Agarose gels on an SV40- cell line showing the efficiency of the RNAi constructs (shFra-1 and shCD44) on knockdown of CD44 and Fra-1 measured by a semi-quantitative RT-PCR reaction and using GAPDH as a control.