Figure 1.

Proliferation, glucose consumption and lactate secretion by NHBE, hT/LT immortalized and hT/LT/Ras-transformed cells. a. Protein extracted from normal human bronchial epithelial cells (NHBE), NHBE cells transduced with telomerase and large T antigen (hT/LT) and subsequently with H-ras^V12 (hT/LT/Ras) was resolved on a 4–20% gradient gel, transferred to a PVDF membrane and expression of human telomerase (hTERT), SV40 large T antigen (LT), H-Ras and α-tubulin was examined by Western blot analysis. b. Protein expression in NHBE, hT/LT and hT/LT/Ras cells by Western blot was quantified by densitometric analyses and expressed as percentage of control (α-tubulin). c. Primary, immortalized and H-Ras^V12-transformed bronchial epithelial cells were cultured in growth media and enumerated after the indicated time periods. d. Unconsumed glucose remaining in the media was measured after 24, 48 and 72 hrs expressed as the (calculated) consumed glucose. e. Lactate secreted into the media by the cells was measured after 24, 48 and 72 hrs. d. Data are expressed as the mean ± SD of four experiments (b, c) and five experiments (d, e).