Figure 3.

a. Cell viability of doxorubicin treated SCC cell lines following pretreatment with anti-miR-98™ and pre-miR-98™. The SCC-4 cell line was grown under normoxia or hypoxia and transfected with anti-miR-98™ or pre-miR-98™. The cell line was then challenged with varying doses of doxorubicin as described in Methods. The surviving fraction of cells as a percentage of control was determined using the MTT assay. Cell lines grown in normoxic conditions revealed greater percent of surviving cells when treated with pre-mir-98™ (●) compared to controls (▼) and anti-miR-98™ (○) transfected cells. (p ≤ 0.05). There was no significant difference between cell lines treated with anti-miR-98™ and controls. Cell lines grown under hypoxic conditions and transfected with pre-miR-98™ (▽) showed a slight increase in surviving cells compared to control cell lines (■) and Anti-miR-98™ (□) transfected cell lines (p ≤ 0.05). There was no significant difference between control and anti-miR-98™ transfected cell lines. Similar results were observed for SCC-9 and SCC-10B cell lines. The error bars represent S.E.M. for 3 experiments. b. Cell viability of cisplantin treated cell lines following pretreatment with anti-miR-98™ and pre-miR-98™. The SCC-4 cell line was grown under normoxic or hypoxic conditions and transfected with anti-miR-98™ while others were transfected with pre-miR-98™. The cell line was then challenged with varying doses of cisplatin as described in Methods. The surviving fraction of cells as a percentage of control was determined using the MTT assay. Cells grown in normoxic conditions revealed greater percent of surviving cells when treated with pre-mir-98™ (▼) compared to controls (●) and anti-miR-98™ (▽) transfected cell lines (p ≤ 0.05). There was no significant difference between cells treated with anti-miR-98™ and controls. Cell lines grown under hypoxic conditions and transfected with pre-miR-98™ (■) showed no significant difference in the percent of surviving cells compared to control cells (○) and Anti-miR-98™ (□) transfected cell lines. Likewise, there was no significant difference between control and anti-miR-98™ transfected cell lines. Similar results were observed for SCC-9 and SCC-10B cell lines. The error bars represents S.E.M. for 3 experiments.