Figure 6.

Expression of LEDGF/p75 mRNA and protein in NB4 cells treated with daunorubicin. A) Expression of LEDGF/p75 mRNA in NB4 cells treated with serum starvation analyzed by RT-PCR. Cell were cultured under serum-free (SF) conditions for 24 and 48 hours before analysis; untreated NB4 cells served as control cells (Ctl). Primers for the rpP2 gene were also included as a control. The RT-PCR results are representative of at least three independent experiments. B) The expression of LEDGF/p75 and ARAP mRNA in NB4 cells exposed for 18 h to daunorubicin (DNR) at 30, 90, or 180 nM. C) Western blot analysis of NB4 cells exposed to DNR as described above. Note the disappearance of full length LEDGF/p75 (75 kDa) and formation of a degradation product * (58 kDa) after exposure to 180 nM DNR.