Figure 5.

Enforced expression of LEDGF/p52b and LEDGF/p75 counteracted leukemic cell death induced by DNR or cAMP analog. The upper panels show differential interference contrast micrographs of IPC-81 leukemia cells with enforced expression of GFP (A-C) or bicistronic expression of LEDGF/p75 and GFP (D-F), and treated 20 h with vehicle (A, D), 10 nM DNR (B, E), or 10 μM 8-pCPT-cAMP (C, F). The lower panel (G) shows % apoptosis (mean ± SEM, n = 5) of IPC cells after 20 h treatment with 10 nM DNR (open bars) or 10 μM 8-pCPT-cAMP (solid bars). Note the lower % apoptosis in cells expressing LEDGF/p75 and LEDGF/p52b than in cells expressing GFP.