Figure 1.

Confirmation of preferential gene expression in chemotherapy-resistant AML. The expression of the genes found by primary differential screening to have the highest relative expression in resistant AML blasts (Table 1) was reinvestigated by RT-PCR of RNA pooled from resistant (R; left column) or low risk (L; right hand column) AML blasts. The level of rpP2, used to normalize expression of the other genes, was also studied. The R-blasts were from patients with relapse from chemotherapy (R1-5) and the L-blasts from chemo-responsive patients (L1-3). See the Material and methods section for further details on patients, cells and RT-PCR procedure.