Research

Effect of BRAF^V600E mutation on transcription and post-transcriptional regulation in a papillary thyroid carcinoma model

Susanne Cahill¹ , Paul Smyth¹ , Karen Denning¹ , Richard Flavin¹ , Jinghuan Li¹ , Astrid Potratz² , Simone M Guenther² , Richard Henfrey² , John J O'Leary¹ and Orla Sheils¹

¹  Dept. of Histopathology, University of Dublin, Trinity College, Dublin, Ireland

²  Applied Biosystems, Foster City, CA, USA

author email corresponding author email

Molecular Cancer 2007, 6:21doi:10.1186/1476-4598-6-21

Published:	13 March 2007

Abstract

Background

microRNAs (miRNAs) are a group of non-coding single stranded RNAs measuring approximately 22 nucleotides in length that have been found to control cell growth, differentiation and apoptosis. They negatively regulate target genes and have recently been implicated in tumourigenesis. Furthermore, miRNA expression profiling correlates with various cancers, with these genes thought to act as both tumour suppressors and oncogenes. Recently, a point mutation in the BRAF gene leading to a V600E substitution has been identified as the most common genetic change in papillary thyroid carcinoma (PTC) occurring in 29–69% of cases. This mutation leads to aberrant MAPK activation that is implicated in tumourigenesis.

Aim

The aim of this study was to identify the effect that BRAF oncogene has on post-transcriptional regulation in PTC by using microRNA analysis.

Results

A unique miRNA expression signature differentiated between PTC cell lines with BRAF mutations and a normal thyroid cell line. 15 miRNAs were found to be upregulated and 23 miRNAs were downregulated. Several of these up/down regulated miRNAs may be involved in PTC pathogenesis. miRNA profiling will assist in the elucidation of disease pathogenesis and identification biomarkers and targets.