Figure 3.

Ad-mda7 activates molecular markers associated with cell killing. Western blot analysis showed activation of PKR, p38MAPK, and pJNK in Ad-mda7-treated tumor cells but not in PBS-treated or Ad-luc-treated tumor cells. We did not observe activation of these markers in normal cells treated with PBS, Ad-luc, or Ad-mda7. β-actin was used as an internal loading control.