Figure 5.

p53 and p73 chimeric proteins. A, Schematic of the Flag-tagged p53 and p73 wild-type (wt) and chimeric proteins. TAD = N-terminal transactivation domain, PRD = proline-rich domain, DBD = DNA-binding domain,, oligo = oligomerization domain, C-term = C-terminal domain. In this diagram, clear (unfilled) areas are from p53, and gray (filled) areas are from p73β. Numbers indicate the amino acid sequence positions in each protein. B, Saos-2 cells were co-transfected with 100 ng pG13-luc DNA and 50 or 100 ng DNA encoding the indicated protein from A. The fold change in pG13-luc activity is plotted compared to the activity of pG13-luc alone, whose value is considered 1.0. Results are the average from two experiments.