Table 1

Different gene mRNA expression on CD133+ cells compared to those on autologous CD133- cells *



No. 66
No. 377
No. 1049
Section
Gene name
CD133-
CD133+
CD133-
CD133+
CD133-
CD133+

A
CD90
1
15.6 ± 0.66
1
12.8 ± 0.94
1
13.5 ± 0.75

CD44
1
5.7 ± 0.48
1
2.5 ± 0.22
1
2.8 ± 0.19

CXCR4
1
337.8 ± 29.2
1
251.5 ± 22.1
1
264.9 ± 22.9

Nestin
1
21.4 ± 1.25
1
23.2 ± 1.65
1
22.1 ± 1.54

MSI
1
84 ± 7.6
1
75.4 ± 7.03
1
53.5 ± 6.2

MELK
1
1351 ± 95.8
1
467.7 ± 40.5
1
514.6 ± 45.6

GLI-1
1
46 ± 3.8
1
43 ± 4.5
1
49 ± 5.9

PTCH
1
16 ± 1.48
1
13.5 ± 0.85
1
14.3 ± 1.24

B
MGMT
1
32.4 ± 2.5
1
34.7 ± 2.9
1
56.3 ± 4.2

BCRP1
1
6.5 ± 0.43
1
4.3 ± 0.25
1
4.8 ± 0.24

SIRT1
1
4.9 ± 0.34
1
4.2 ± 0.26
1
5.4 ± 0.29

FLIP
1
294 ± 25.5
1
157.6 ± 14.2
1
145.6 ± 13.7

BCL-2
1
13.9 ± 0.95
1
4.9 ± 0.54
1
3.8 ± 0.54

BCL-XL
1
5.6 ± 0.39
1
3.2 ± 0.16
1
2.5 ± 0.14

cIAP1
1
39.0 ± 3.5
1
4.3 ± 0.53
1
5.6 ± 0.65

cIAP2
1
3 ± 0.25
1
1.9 ± 0.12
1
1.7 ± 0.14

XIAP
1
21.9 ± 2.2
1
9.7 ± 0.68
1
10.3 ± 0.91

NAIP
1
12.1 ± 0.75
1
6.4 ± 0.43
1
4.5 ± 0.62

Survivin
1
1.6 ± 0.08
1
2.3 ± 0.18
1
2.4 ± 0.18

BAX
1
0.33 ± 0.03
1
0.49 ± 0.06
1
0.21 ± 0.05

* Total RNA was extracted from both CD133+ and CD133- fraction after FACS sorting from three primary cultured cells (No. 66, 377 and 1049). Different gene mRNA expression was measured by real-time qPCR. The relative mRNA expression of different gene in CD133+ cells was presented as the fold increase compared to that of autologous CD133- cells (see Methods). Data was presented as Mean ± SD of three independent experiments.

Liu et al. Molecular Cancer 2006 5:67   doi:10.1186/1476-4598-5-67