Figure 3.

Electropherogram of PCR with microsatellite IVS27AC28. A: PCR with DNA from peripheral blood of the father of the patient. B: PCR with DNA from peripheral blood of the patient. M indicates the maternal allele. C,D: PCR with DNA from microdissected melanoma cells showing LOH of the maternal allele (C) and a PCR product of reduced size (D, asterisk). Dotted lines indicate peaks and sizes of the molecular size marker GeneScan^®-400 HD [ROX].