Table 2

Genes identified by Prediction Analysis of Microarrays (PAM) differentially expressed in normal skin compared to non-melanoma skin cancer.
A) Up-regulated genes (42) in non-melanoma skin cancer.							Microarray experiments			ANOVA

	No.	Accession No.	Symbol	Gene	Function	Localization	Change fold T/N	Mean of raw signal (normal)	Mean of raw signal (AK&SCC)	p-value

Differentiation
	1	AF183421.1	rab22b	Small GTP-bindung protein rab22 (RAB31)	Small GTPase signal transduction.	18p11.3	1.87	6853	12495	n.s.
	2	NM_006868.1	RAB31*	RAB31, member RAS oncogene family	Small GTPase signal transduction.	18p11.3	3.30	2637	8437	0.027
Apoptosis
	3	NM_004834.1	MAP4K4*	Mitogen-activated protein kinase kinase kinase kinase 4	A member of the serine/threonine protein kinase family, specifically activate MAPK8/JNK.	2q11.2-q12	2.65	1475	3898	0.024
Proliferation
	4	NM_007375.1	TARDBP	TAR DNA binding protein	Transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.	1p36.22	1.48	6779	10441	n.s.
Metabolism
	5	D55674.1	hnRNP D	Heterogeneous nuclear ribonucleoprotein D	Associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.	4q21.1-q21.2	1.64	4020	6968	n.s.
	6	NM_003942.1	RPS6KA4	Ribosomal protein S6 kinase	RSK (ribosomal S6 kinase) family of serine/threonine kinases	11q11-q13	1.74	3374	6029	0.044
	7	NM_001814.1	CTSC	Cathepsin C (CTSC)	Defects in the encoded protein have been shown to be a cause of Papillon-Lefevre syndrome, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis.	11q14.1-q14.3	1.64	11435	18686	n.s.
	8	Z14077.1	YY1	YY1, NF-E1 protein	Transcription factor involved in repressing and activating a diverse number of promoters.	14q	2.08	4185	8939	n.s.
	9	NM_006141.1	DNCLI2	Dynein, cytoplasmic, light intermediate polypeptide 2	Involved in retrograde organelle transport and some aspects of mitosis.	16q22.1	3.23	2225	5692	n.s.
	10	AF061832.1	M4	M4 protein deletion mutant	Appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.	19p13.3-p13.2	1.29	11154	14824	n.s.
Cell communication
	11	U65590	IL-1RN*	IL-1 receptor antagonist IL-1Ra	Inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.	2q14.2	1.75	9599	18079	n.s.
	12	NM_004688.1	NMI*	N-myc (and STAT) interactor	Interacts with NMYC, CMYC, all STATs except STAT2.	2p24.3-q21.3	2.33	2744	6248	n.s.
	13	NM_002416.1	MIG	Monokine induced by gamma interferon	Binding to CXCR3 causes pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression.	4q21	3.54	1492	10529	n.s.
	14	U72069.1	TNPO1	Karyopherin beta2	Interacts with nuclear localization signals to target nuclear proteins to the nucleus.	5q13.2	2.06	2986	6049	n.s.
	15	BC004489.1	HLA-C	Major histocompatibility complex, class I, C	A central role in the immune system by presenting peptides derived from ER lumen.	6p21.3	1.30	78196	106054	n.s.
	16	L42024.1	HLA-B39	MHC, HLA-B39	A central role in the immune system by presenting peptides derived from ER lumen.	6p21.3	1.35	78040	114101	n.s.
	17	NM_005516.1	HLA-E	Major histocompatibility complex, class I, E	A central role in the immune system by presenting peptides derived from ER lumen.	6p21.3	1.76	24252	43847	n.s.
	18	NM_006096.1	NDRG1	N-myc downstream regulated gene 1	Involved in stress responses, hormone responses, cell growth, and differentiation.	8q24.3	2.60	17233	44914	0.024
	19	AF313468.1	lectin-1	Dendritic cell-associated C-type lectin-1	Diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response.	12p12.3-p13.2	3.61	1163	4146	0.019
	20	U88964	HEM45	HEM45	HIV-1 Tat upregulates the interferon-responsive gene expression of HEM45.	15q26	3.24	982	3293	0.019
	21	NM_000418.1	IL4R*	Interleukin 4 receptor	Development of allergic reactions and have been shown to modulate the function of monocytes and macrophages.	16p11.2-12.1	2.41	2519	6159	0.014
	22	NM_015986.1	CREME9	Cytokine receptor-like molecule 9	Thought to be involved in signal transduction.	17q11.2	1.58	4335	6950	0.015
	23	NM_002087.1	GRN*	Granulin	A role in the development of prostatic intraepithelial neoplasia.	17q21.32	1.77	14453	25978	0.034
Adhesion
	24	AK023406.1	FLJ13344	FLJ13344 fis	High homology to the actin and microtubules binding protein ABP620.	1p32-p31	2.49	3656	9107	0.038
	25	U03271	CAPZB	F-actin capping protein beta subunit	Regulates growth of the actin filament by capping the barbed end of growing actin filaments.	1p36.1	1.35	11809	16060	n.s.
	26	NM_005572.1	LMNA	Lamin AC	Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression.	1q21.2-q21.3	1.47	13531	19844	n.s.
	27	AB010427.2	WDR1	NORI-1	May help induce the disassembly of actin filaments.	4p16.1	2.11	7418	15927	<0.001
	28	NM_004893.1	H2AFY	H2A histone family, member Y	A member of the histone H2A family.	5q31.3-q32	1.39	18975	26758	0.048
	29	NM_001101.2	ACTB	Actin, beta	Conserved proteins that are involved in cell motility, structure and integrity.	7p15-p12	1.44	120864	178342	0.026
	30	NM_000700.1	ANXA1	Annexin A1	Located on the cytosolic face of the plasma membrane.	9q12-q21.2	1.74	35910	64023	0.043
	31	NM_002160.1	TNC*	Hexabrachion (tenascin C, cytotactin) (HXB),	Spliced tenascin-C has important roles in tumor progression of breast cancer.	9q33	4.60	1819	8694	0.038
	32	BF338947	1–8U	Interferon induced transmembrane protein 3	Transmembrane protein.	11p15.5	1.70	20588	33223	n.s.
	33	NM_002421.2	MMP1*	Matrix metalloproteinase 1	Involved in the breakdown of extracellular matrix in normal physiological processes (embryonic development, reproduction, and tissue remodeling), in disease processes (arthritis, metastasis).	11q22.3	> 10	293	18006	0.024
	34	J00269.1	KRT6A	Human 56 k cytoskeletal type II keratin	Member of the keratin gene family.	12q12-q13	3.79	42604	161154	0.026
	35	NM_006825.1	CKAP4	Transmembrane protein	Required for cell adhesion.	12q24.11	1.68	12780	22154	0.023
	36	NM_005561.2	LAMP1	Lysosomal-associated membrane protein 1	Possible a role in tumor cell metastasis.	13q34	1.28	25495	33858	n.s.
	37	NM_001793.1	CDH3	Cadherin 3, type 1, P-cadherin (placental)	Calcium-dependent cell-cell adhesion glycoprotein, mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy.	16q22.1	3.63	3819	15934	0.043
	38	NM_004360.1	CDH1*	Cadherin 1, type 1, E-cadherin (epithelial)	Calcium dependent cell-cell adhesion glycoprotein, mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer.	16q22.1	1.31	18773	24888	n.s.
	39	BC001920.1	actin gamma	Actin, gamma 1	Involved in various types of cell motility, and maintenance of the cytoskeleton.	17q25	1.38	108509	152446	n.s.
	40	NM_001614.2	ACTG1	Actin, gamma 1	Involved in various types of cell motility, and maintenance of the cytoskeleton.	17q25	1.43	113681	166361	n.s.
	41	NM_004368.1	CNN2	Calponin 2	Participates in smooth muscle contractio, cell adhesion and can bind actin.	21q11.1	1.43	4012	5822	n.s.
	42	NM_004994.1	MMP9	Matrix metalloproteinase 9 (gelatinase B, collagenase)	Involved in the breakdown of extracellular matrix in normal physiological processes (embryonic development, reproduction, tissue remodeling), in disease processes (arthritis and metastasis).	20q11.2-q13.1	4.70	3150	16961	n.s.

B) Down-regulated genes (76) in non-melanoma skin cancer.							Microarray experiments			ANOVA

	No.	Accession No.	Symbol	Gene	Function	Localization	Change fold N/T	Mean of raw signal (normal)	Mean of raw signal (AK&SCC)	p-value

Differentiation
	1	NM_004430.1	EGR3	Early growth response 3	Participates in the transcriptional regulation of genes in controling biological rhythm.	8p23-p21	2.22	7833	3782	n.s.
	2	NM_022969.1	FGFR2	Fibroblast growth factor receptor 2	Influences mitogenesis and differentiation.	10q26	2.04	9967	5011	n.s.
	3	BC001971.1	p27, Kip1	Cyclin-dependent kinase inhibitor 1B	Binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1.	12p13.1-p12	1.45	8234	5959	n.s.
	4	AF309553.1	REC14	Meiotic recombination protein REC14	Exact function unknown. Thought to be involved in differentiation.	15q24.1	1.96	16148	8395	0.027
	5	NM_001424.1	EMP2	Epithelial membrane protein 2	A role in the control of cell proliferation, cell differentiation, and cell death.	16p13.2	2.00	21074	11007	0.026
	6	NM_001983.1	ERCC1*	Excision repair cross-complementing rodent repair deficiency	Repair protein expression is reduced in testis neoplasms.	19q13.2-q13.3	1.79	7222	4073	n.s.
Apoptosis
	7	NM_016085.1	APR-3^n.s.	Apoptosis related protein APR-3	Involved in apoptosis, and may also be involved in hematopoietic development and differentiation.	2p23.3	1.85	21640	11863	0.012
	8	AB055804.1	MM-1 beta	MM-1 beta	Assists in the correct folding of other proteins, subunit is thought to repress the transcriptional activity of c-myc.	12q12	1.64	35682	22035	n.s.
	9	NM_015965.1	CGI-39*	CGI-39 protein; cell death-regulatory protein GRIM19	Signal transducer and activator of transcription 3.	19p13.2	1.43	12888	9485	n.s.
Proliferation
	10	BC003689.1	HMG 17	High-mobility group (non-histone chromosomal protein 17)	Nucleosomal binding protein.	1p36.1	1.22	46229	38384	n.s.
	11	NM_006694.1	JTB	Jumping translocation breakpoint	Up-regulation in hepatocellular carcinoma (HCC).	1q21	1.39	26313	19283	0.030
	12	NM_001967.2	EIF4A2	Eukaryotic translation initiation factor 4A	Protein translation.	3q28	1.92	41342	22341	0.019
	13	NM_005935.1	MLLT2	Myeloidlymphoid or mixed-lineage leukemia	Interaction with SIAH1 and SIAH2 proteins, the cell cycle control exerted by MLL-AF4 may be responsible of resistance to cell-death.	4q21	1.82	14146	8083	n.s.
	14	AF279903.1	EC45	60S ribosomal protein L15	A ribosomal protein that is a component of the 60S subunit.	3p24.1	1.85	60783	36597	n.s.
	15	NM_002948.1	RPL15	Ribosomal protein L15	A ribosomal protein that is a component of the 60S subunit, overexpressed in some esophageal tumors compared to normal matched tissues.	3p24.1	1.79	79109	46326	n.s.
	16	NM_001023.1	RPS20	Ribosomal protein S20	A ribosomal protein that is a component of the 40S subunit.	8q12	1.35	78126	58507	n.s.
	17	NM_000995.1	RPL34	Ribosomal protein L34	Component of the 60S subunit.	4q25	1.85	92356	52954	n.s.
	18	NM_006098.1	GNB2L1	Guanine nucleotide binding protein (G protein)	Regulates G1/S progression by suppressing Src kinase activity.	5q35.3	1.32	73713	57754	n.s.
	19	NM_007104.2	RPL10A	Ribosomal protein L10a	Downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug.	6p21.3-p21.2	1.49	77178	54218	n.s.
	20	BC000734.1	EIF3S6	Eukaryotic translation initiation factor 3	Protein translation.	8q22-q23	1.38	51705	37233	n.s.
	21	NM_003756.1	EIF3S3	Eukaryotic translation initiation factor 3	Protein translation, overexpression of EIF3S3 is associated with breast and prostate cancer.	8q24.11	1.49	38725	25795	n.s.
	22	BC000524.1	RPS6	Ribosomal protein S6	Protein synthesis, cytoplasmic ribosomal protein that is a component of the 40S subunit.	9p21	1.23	99499	83221	n.s.
	23	NM_001417.1	EIF4B	Eukaryotic translation initiation factor 4B	Stimulate the nuclease activity of herpes simplex virus.	12q13.13	1.35	15184	11509	n.s.
	24	NM_000968.1	RPL4	Ribosomal protein L4	Component of the 60S subunit.	15q22	1.30	118180	92600	n.s.
	25	NM_015920.1	LOC51065	40S ribosomal protein S27	Similarity with ribosomal protein S27.	15q22.1	1.67	24852	15517	0.043
	26	NM_001009.1	RPS5	Ribosomal protein S5	Variable expression of this gene in colorectal cancers compared to adjacent normal tissues, although no correlation between the severity of this disease has been observed.	19q13.4	1.54	57497	38110	0.033
	27	NM_016091.1	HSPC025	HSPC025	Interacts with Int-6 and is associated with eukaryotic translation initiation factor 3.	22q	1.45	58905	41562	0.043
	28	L22453.1	TARBP-b	HIV-1 TAR RNA binding protein (RPL3)	Encodes a ribosomal protein that is a component of the 60S subunit; the protein can bind to the HIV-1 TAR mRNA, and the protein probably contributes to tat-mediated transactivation.	22q13	1.47	86865	59742	n.s.
	29	NM_006013.1	RPL10	Ribosomal protein L10	This gene encodes a ribosomal protein that is a component of the 60S subunit.	Xq28	1.27	127604	102770	n.s.
	30	NM_014315.1	LCP	host cell factor homolog	Transcriptional coactivator which coordinates the assembly of enhancer complex through direct interactions with viral and cellular trans-activators.	14q21.3-q22.1	1.92	12588	6756	0.003
Metabolism
	31	NM_015958.1	LOC51611	CGI-30 protein	Activities of Dipthine synthase, methyltransferase, and transferase.	1p21.2	1.67	7739	4726	n.s.
	32	NM_000847	GSTA3	Glutathione S-transferase A3	Involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds.	6p12.1	5.00	1557	135	0.005
	33	NM_002633.1	PGM1	Phosphoglucomutase 1	Regulatory enzyme in cellular glucose utilization and energy homeostasis.	1p31	2.22	22301	10170	0.044
	34	BC006229.1	CYC5b	Cytochrome c oxidase subunit Vb	Terminal enzyme of the mitochondrial respiratory chain.	2cen-q13	2.00	17780	9105	0.015
	35	NM_001862.1	COX5B	Cytochrome c oxidase subunit Vb	Terminal enzyme of the mitochondrial respiratory chain.	2cen-q13	1.85	26820	14737	0.035
	36	NM_002492.1	NDUFB5	NADH dehydrogenase (ubiquinone) 1 beta subcomplex	A subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I).	3q27.1	1.67	18898	11821	n.s.
	37	NM_021122.2	FACL2	Fatty-acid-Coenzyme A ligase	Plays a role in lipid biosynthesis and fatty acid degradation.	4q34-q35	2.50	39142	15864	n.s.
	38	BC005270.1	ND4FS4	NADH dehydrogenase (ubiquinone) Fe-S protein 4	Multisubunit enzyme complex of the mitochondrial respiratory chain, plays a vital role in cellular ATP production.	5q11.1	1.85	10920	5956	n.s.
	39	NM_001867.1	COX7C	Cytochrome c oxidase subunit VIIc	Mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen.	5q14	2.17	55286	26201	0.013
	40	BC003674.1	NDUFA2	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex	Multisubunit enzyme complex of the mitochondrial respiratory chain, plays a vital role in cellular ATP production.	5q31	1.67	9562	5854	n.s.
	41	NM_014402.1	QP-C	Low molecular mass ubiquinone-binding protein	Activities of oxidoreductase, and ubiquinol-cytochrome c reductase.	5q31.1	1.69	32210	19064	n.s.
	42	BC001917.1	MDH2	Malate dehydrogenase 2, NAD (mitochondrial)	Oxidation of malate to oxaloacetate (citric acid cycle).	7p12.3-q11.2	1.30	24490	19165	n.s.
	43	NM_002489.1	NDUFA4	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex	Multisubunit enzyme complex of the mitochondrial respiratory chain, plays a vital role in cellular ATP production.	7p21.3	1.59	22038	14006	n.s.
	44	NM_005004.1	NDUFB8	NADH dehydrogenase (ubiquinone) 1 beta subcomplex	Multisubunit enzyme complex of the mitochondrial respiratory chain.	10q23.2-q23.33	1.41	25375	18105	n.s.
	45	NM_004074.1	COX8	Cytochrome c oxidase subunit VIII	Terminal enzyme of the respiratory chain.	11q12-q13	1.37	21360	15941	n.s.
	46	NM_004549.1	NDUFC2	NADH dehydrogenase (ubiquinone) 1	Multisubunit enzyme complex of the mitochondrial respiratory chain.	11q13.4	1.59	33921	21642	n.s.
	47	AF042165	COX7CP1	Cytochrome c oxidase subunit VIIc	Terminal component of the mitochondrial respiratory chain.	13q14-q21	2.08	40375	19940	0.003
	48	NM_001861.1	COX4	Cytochrome c oxidase subunit IV	Mitochondrial respiratory chain.	16q22-qter	1.25	54196	44561	n.s.
	49	L47162.1	FALDH	Human fatty aldehyde dehydrogenase	Detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.	17p11.2	2.50	16420	6898	0.038
	50	NM_000382.1	ALDH3A2	Aldehyde dehydrogenase 3 family	Detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.	17p11.2	3.33	19513	5867	0.020
	51	NM_021074.1	NDUFV2	NADH dehydrogenase (ubiquinone) flavoprotein 2	Activities of NADH dehydrogenase, electron carrier, and oxidoreductase.	18p11.31-p11.2	1.82	20847	11262	n.s.
	52	M22865.1	CYb5	Human cytochrome b5	Cytochrome c oxidase activity.	18q23	3.57	36175	11654	n.s.
	53	M22976.1	cyt b5	Human cytochrome b5, 3 end	Cytochrome c oxidase activity.	18q23	3.70	22288	6090	n.s.
	54	NM_001914.1	CYB5	Cytochrome b-5	Cytochrome c oxidase activity.	18q23	3.33	31694	10220	n.s.
	55	NM_013387.1	HSPC051	Ubiquinol-cytochrome c reductase complex	Activities of oxidoreductase, and ubiquinol-cytochrome c reductase.	22cen-q12.3	1.43	15592	11200	n.s.
	56	NM_001866.1	COX7B	Cytochrome c oxidase subunit VIIb	Encoded by COX, the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen.	Xq13.3	1.37	29860	21796	n.s.
	57	NM_004541.2	NDUFA1	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex	Codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone.	Xq24	2.27	25486	11413	0.026
Cell communication
	58	BC002669.1	NR2	Nuclear receptor subfamily 2	Predicted to encode proteins that are very similar in primary structure to receptors for steroid hormones or thyroid hormone (T3).	19p13.1	1.51	7213	4917	n.s.
	59	NM_004182.1	UXT	Ubiquitously-expressed transcript	Interacts with the N-terminus of the androgen receptor and plays a role in facilitating receptor-induced transcriptional activation.	Xp11.23-p11.22	1.59	11609	7489	n.s.
	60	M92439.1	LeuP	Human leucine-rich protein	Activate expression of MDR1 and MVP (key components of the cytotoxic defense network).	2p21	1.92	6429	3379	n.s.
Adhesion
	61	NM_006893.1	LGTN	Ligatin	A role in neuroplasticity by modulating cell-cell interactions, intracellular adhesion, and protein binding at membrane surfaces.	1q31-q32	1.70	9444	5566	n.s.
	62	M80927.1	CHI3L1**	Human glycoprotein -39	Extracellular matrix structural constituent, sugar binding, and hydrolase activity.	1q32.1	7.69	31265	5550	n.s.
	63	NM_015717.1	LANGERIN	Langerhans cell specific c-type lectin	Expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa.	2p13	4.00	4586	1323	0.023
	64	NM_007234.2	DCTN3	Dynactin 3 (p22)	Involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis.	9p13	1.45	13690	9694	n.s.
	65	NM_018663.1	LOC55895	Peroxisomal membrane protein	Peroxisome organization and biogenesis (assembly and arrangement of peroxisome).	12q24.33	3.45	6928	2170	n.s.
Detoxification
	66	NM_001512.1	GSTA4	Glutathione S-transferase A4	Involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds.	6p12.1	1.41	25838	18009	n.s.
	67	NM_004528.1	MGST3	Microsomal glutathione S-transferase 3	Involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation.	1q23	1.96	22933	12007	0.023
	68	NM_002413.1	MGST2	Microsomal glutathione S-transferase 2	Catalyzes the conjugation of leukotriene A4 and reduced glutathione to produce leukotriene C4.	4q28.3	1.82	10888	6065	n.s.
	69	NM_015917.1	LOC51064	Glutathione S-transferase subunit 13 homolog	Activities of glutathione transferase, protein disulfide oxidoreductase, and transferase.	7	1.75	9422	5458	n.s.
	70	NM_001752.1	CAT	Catalase	Abnormal expression of catalase in the eutopic and ectopic endometrium strongly suggests pathologic involvement of free radicals in endometriosis and adenomyosis.	11p13	1.61	27669	17923	n.s.
	71	L19185	NKEFB^n.s.	Human natural killer cell enhancing factor	Reduce hydrogen peroxide and alkyl hydroperoxides.	19p13.2	2.50	39723	17286	n.s.
unknown
	72	NM_016098.1	LOC51660	HSPC040 protein	Unknown Function.	6q27	3.33	15481	4682	0.038
	73	AL356115	KIAA1128	KIAA1128 protein	Unknown Function.	10q23.2	1.38	126962	93488	0.048
	74	AK022248	FLJ12186	FLJ12186	Unknown Function.	14q22.3	1.38	15261	11116	n.s.
	75	NM_004868.1	GPSN2	Glycoprotein, synaptic 2	Unknown Function.	19p13.12	1.69	19830	11888	n.s.
	76	AF151056.1	HSPC222	HSPC222	Unknown Function.	unknown	1.59	40513	26566	0.030

The accession number, the symbol, the description of the genes, their function, their chromosome localization, the change fold, the raw signal (mean value), and the p values of the ANOVA analysis are shown. The accession numbers in bold represent the 42 genes identified by PAM and ANOVA (p < 0.05), which were significantly differentially expressed. The symbols marked in bold represent the genes verified by quantitative real-time RT-PCR. A) Up-regulated genes (42) in non-melanoma skin cancer. B) Down-regulated genes (76) in non – melanoma skin cancer. * significant expression difference verified by quantitative real-time RT-PCR. ** positive with two different affymetrix numbers (209395_at and 209396_s_at). No., numbers; T, Tumor (AK and SCC), N, normal skin; AK, actinic keratosis; SCC, squamous cell carcinoma; n.s., not significant.
Nindl et al. Molecular Cancer 2006 5:30 doi:10.1186/1476-4598-5-30