Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies

doi:10.1186/1476-4598-5-24

Molecular Cancer

Volume 5

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Mattie MD
Benz CC
Bowers J
Sensinger K
Wong L
Scott GK
Fedele V
Ginzinger D
Getts R
Haqq C

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Benz CC
Bowers J
Sensinger K
Wong L
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Fedele V
Ginzinger D
Getts R
Haqq C
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Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies

Michael D Mattie¹ , Christopher C Benz⁴ , Jessica Bowers² , Kelly Sensinger² , Linda Wong³ , Gary K Scott⁴ , Vita Fedele¹ , David Ginzinger³ , Robert Getts² and Chris Haqq¹

¹UCSF Comprehensive Cancer Center, Department of Urology, San Francisco, California 94115, USA

²Genisphere Inc., Hatfield, Pennsylvania 19440, USA

³Applied Biosystems, Foster City, California 94404, USA

⁴Buck Institute for Age Research, Novato, California 94945, USA

author email corresponding author email

Molecular Cancer 2006, 5:24doi:10.1186/1476-4598-5-24


Published:	19 June 2006

Additional files

Additional File 1:

Schematic of miRNA labeling procedure.

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Additional File 2:

Fidelity of labeling and amplification procedures in microarray analysis.

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Additional File 3:

Raw data of qRT-PCR and microarray data for PC3vs. LNCaP comparison.

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Additional File 4:

miRNA expression in ErbB2+ tumors vs. SKBr3 cell line.

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Additional File 5:

Clinical annotation for breast tumor specimens.

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Additional File 6:

Oligonucleotide sequences printed on microarrays.

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Additional File 7:

qRT-PCR data of let-7 and miR-16 in PC3 and LNCaP cells. Includes PCR efficiency data.

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Additional File 8:

Raw data for microarrays of clinical prostate specimens

Format: XLS Size: 46KB Download file

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Additional File 9:

Raw data for microarrays of clinical breast tumor tissues.

Format: XLS Size: 161KB Download file

This file can be viewed with: Microsoft Excel Viewer