Figure 3.

Immunoperoxidase staining of HLTF in kidney and tumor tissue. A-D, kidneys from DES-treated hamsters; E-F, HKT-1097-derived tumor xenograft. Tissue sections were processed for HLTF immunostaining with antisera directed against either HLTFMet123 (ASE2; A, B, D) or HLTFMet1 (ART2; C, E, F). A, HLTF immunolabeling associated with early signs of neoplastic transformation (tumorous bud; two months of DES exposure). B, C, scattered HLTF-positive cells in large renal tumors (6 months of DES exposure). D, cell populations with HLTF immunoreactivity in a large tumor (11 months of DES exposure). E, F, scattered HLTF-positive cells in subcutaneous tumor xenograft.