Figure 2.

EGFR is unaltered in C/EBPbeta-2 overexpressing MCF10As. Uninfected MCF10A cells (lanes 1 & 2), sorted MCF10A cells infected with LZRS-GFP virus (lanes 3 & 4), and subcultured MCF10A cells infected with LZRS-His-C/EBPbeta-2-IRES-GFP virus (lanes 5 & 6) were grown in the presence (lanes 1, 3, and 5) or absence (lanes 2, 4, and 6) of EGF for 3 days. Cell extracts were subjected to 8% SDS PAGE and blotted with an antibody that specifically detect (A) phosphorylated tyrosine 1173 (pY1173 EGFR). The same blot was reblotted with an antibody that detects total EGFR for normalization (B).