C/EBPbeta-2 confers EGF-independent growth and disrupts the normal acinar architecture of human mammary epithelial cells

Additional File 1:

Six optical sections of acini comprised of MCF10A cells infected with LZRS-GFP virus show the cell nuclei stained with ethidium homodimer-2 (red) and enhanced GFP expression (green). Image planes progress from the surface of the sphere (Supp. 7a1), downward toward the center (Supp. 7a3), to the opposite surface (Supp. 7a6). Sections are imaged every 9 microns.

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Additional File 2:

Eighteen optical sections of acini comprised of MCF10A cells infected with LZRS-GFP virus illustrate f-actin organization stained with Alexa 594 conjugated phalloidin (red) and enhanced GFP expression (green). Image planes progress from the focal plane near the surface (Supp 7b1), downward toward the center (Supp 7b9), to the opposite surface (Suppl 7b18). Sections are imaged every 3 microns.

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Additional File 3:

Eight optical sections of acini comprised of MCF10A cells infected with LZRS-His-C/EBPbeta-2-IRES-GFP virus show the cell nuclei stained with ethidium homodimer-2 (red) and enhanced GFP expression (green). Image planes progress from the surface of the sphere (Supp 7c1), downward toward the center (Supp. 7c4), to the opposite surface (Supp. 7c8). Sections are imaged every 9 microns.

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Additional File 4:

Montage of sixteen optical sections of acini comprised of MCF10A cells infected with LZRS-His C/EBPbeta-2-IRES-GFP virus illustrate the lack of f-actin organization stained with Alexa 594-conjugated phalloidin (red) and enhanced GFP expression (green). Image planes progress from the focal plane near the surface (Supp 7d1), downward toward the center (Supp 7d8), to the opposite surface (Supp 7d16). Sections are imaged every 3 microns.

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Additional File 5:

Montage of eleven optical sections of acini comprised of MCF10A cells infected with LZRS GFP virus show organization of cell nuclei to form a hollow sphere. The nuclei are stained with ethidium homodimer-2 (red). Image planes progress from the focal plane near the surface (Supp 8a1), downward toward the center (Supp 8a5), to the opposite surface (Supp 8a11). The cells express GFP (not shown).

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Additional File 6:

Montage of sixteen optical sections of acini comprised of MCF10A cells infected with LZRS-His-C/EBPbeta-2-IRES-GFP virus show disorganized cell nuclei that fill the mammosphere. The nuclei are stained with ethidium homodimer-2 (red). Image planes progress from the focal plane near the surface (Supp 8b1), downward toward the center (Supp 8b8), to the opposite surface (Supp 8b16). Although not shown, the cells also express GFP.

Format: MOV Size: 511KB Download file

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Additional File 7:

Montage of twenty optical sections of acini comprised of MCF10A cells infected with LZRS-His-C/EBPbeta-2-IRES-GFP virus show disorganized cell nuclei that fill the mammosphere. The nuclei are stained with ethidium homodimer-2 (red). Image planes progress from the focal plane near the surface (Supp 8c1), downward toward the center (Supp 8c10), to the opposite surface (Supp 8c20). Although not shown, the cells also express GFP.

Format: MOV Size: 857KB Download file

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