Figure 7.

(A-B). Effects of Hsulf-1 on EGF/HB-EGF and FGF-2 induced receptor phosphorylation and MAPK phosphorylation. Panc-1 pancreatic cancer cells were cultured in 1% FBS medium overnight and then incubated with 10 μg/ml of the indicated growth factors for 10 min. Phosphorylation of MAP kinase and receptor phosphorylation (EGFR) was determined by immunoblotting with antibodies specific for phospho-p44/42 MAPK and phospho-EGFR. Equal loading was determined by re-blotting the membranes with an γ-tubulin antibody. The figure is representative of three independent experiments. (C) Hsulf-1 attenuates FGF-2 stimulated invasion. An in vitro cell invasion assay was performed using 8 μM filters coated with Matrigel as described in the Material and Methods section. Panc-1 cells (1.25 × 10⁵) were seeded onto the filters in 1% serum overnight, and then treated as indicated for 24 h. The values shown are the mean ± SEM obtained from three independent experiments.