Figure 4.

RAR reciprocally regulates anti-metastatic TIMP 1 and pro-metastatic MMP 9 in a ligand dependent and reversible manner. MMP 9 and TIMP 1 gene expression in WT-Line 1 tumor cells exposed to at-RA +/- AGN193109 for 24 hours at the indicated concentrations was assessed by real time PCR. The results represent the average of 3 independent experiments, normalized to β-actin loading controls.