Figure 3.

Bar graphs indicating percentage of increase of intracellular free [Ca²⁺] using fura-2. These data were generated from U87MG cells grown in phenol red-free medium for 24 h prior to treatments with the drugs in freshly prepared phenol red-free medium. Four treatment groups: control (CTL); 40 μM dexamethasone (DXM) for 8 h; 100 μM temozolomide (TMZ) for 6 h; pretreatment with 40 μM DXM for 2 h followed by 100 μM TMZ for 6 h. Percent changes in intracellular free [Ca²⁺] were shown at nM levels. Significant difference between CTL and TMZ treated cells was indicated by * (P ≤ 0.05) and significant difference between TMZ treated cells and DXM plus TMZ treated cells was indicated by # (P ≤ 0.05).