Genetic alterations and in vivo tumorigenicity of neurospheres derived from an adult glioblastoma

doi:10.1186/1476-4598-3-25

Molecular Cancer

Volume 3

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Tunici P
Bissola L
Lualdi E
Pollo B
Cajola L
Broggi G
Sozzi G
Finocchiaro G

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Tunici P
Bissola L
Lualdi E
Pollo B
Cajola L
Broggi G
Sozzi G
Finocchiaro G
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Short communication

Genetic alterations and in vivo tumorigenicity of neurospheres derived from an adult glioblastoma

Patrizia Tunici¹ , Lorena Bissola¹ , Elena Lualdi² , Bianca Pollo³ , Laura Cajola¹ , Giovanni Broggi⁴ , Gabriella Sozzi² and Gaetano Finocchiaro¹

¹Istituto Nazionale Neurologico Besta, Dept. Experimental Neurology, Milano, Italy

²Istituto Nazionale Tumori, Dept. Experimental Oncology, Milano, Italy

³Istituto Nazionale Neurologico Besta, Dept. Clinical Neurosciences, Milano, Italy

⁴Istituto Nazionale Neurologico Besta, Dept. Neurosurgery; Milano, Italy

author email corresponding author email

Molecular Cancer 2004, 3:25doi:10.1186/1476-4598-3-25


Published:	6 October 2004

Abstract

Pediatric brain tumors may originate from cells endowed with neural stem/precursor cell properties, growing in vitro as neurospheres. We have found that these cells can also be present in adult brain tumors and form highly infiltrating gliomas in the brain of immunodeficient mice. Neurospheres were grown from three adult brain tumors and two pediatric gliomas. Differentiation of the neurospheres from one adult glioblastoma decreased nestin expression and increased that of glial and neuronal markers. Loss of heterozygosity of 10q and 9p was present in the original glioblastoma, in the neurospheres and in tumors grown into mice, suggesting that PTEN and CDKN2A alterations are key genetic events in tumor initiating cells with neural precursor properties.