Figure 6.

Long term effects of Aurora-A and Cyclin D1 co-expression. (A) Logarithmically growing Wi-38 cells were infected with the adenoviruses indicated. Three days after infection, cells were analyzed by PI-staining. One of two data sets is presented. (B) A growth curve experiment was performed with logarithmically growing Wi-38 infected with the viruses indicated. Data of two independent experiments are shown. (C) Serum starved Wi-38 cells were infected with adenoviruses expressing control (lacZ), Aurora-A, Cyclin D1 or a combination of these proteins. Cells were induced by serum addition and analyzed by PI-staining after 48 hours. A representative of three experiments is depicted. (D) Wi-38 cells were treated as in (C), but infected with the indicated combinations of Aurora-A, Cyclin D1 and p53^V143A expressing adenoviruses. After 48 and 72 hours, cells were analyzed by PI-staining. Bars depict the percentage of cells with a DNA content >4N. A representative of two experiments is shown.