Figure 3.

PKR mediates cytokine production by reovirus treated melanoma cells. (A) Melanoma cell lines were seeded in 24 well plates and pre-treated with 2.5 mM 2-AP or equivalent PBS controls, for 2 hours prior to addition of reovirus at the indicated doses. Supernatants were collected after 48 hours and IL-8 levels determined. Data are representative of at least 3 independent experiments. * indicates P < 0.05, by Student's t-test. (B) Mel-624 tumour cells were seeded in 100 mm dishes and transfected with 100 nM PKRV or irrelevant control siRNA. Cell lysates were prepared and western blotted for total PKR, with β-actin used to confirm equal track loading. (C) Mel-624 cells were seeded in 24 well plates and transfected with 100 nM PKRV or irrelevant control siRNA; reovirus was then added at the indicated doses. Supernatants were collected 24 and 48 hours later and IL-8 levels determined. Data are representative of two independent experiments. * indicates P < 0.05 by Student's t-test.